Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Reaction of rhododendrol glycoside hydrolysis catalyzed by β-glucosidase and RAPIDASE.

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques:

Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Hydrolysis of betuloside and apiosylrhododendrin mixture ( T = 40 °C; 100 mM phosphate buffer, pH 6). Correlation of experimental data and model through changes in the concentration of betuloside, apiosylrhododendrin, and rhododendrol over time in a batch reactor: (A) c betuloside = 64.5 mM, c apiosylrhododendrin = 16.6 mM, γ RAPIDASE = 20.0 mg mL –1 , (B) c betuloside = 62.3 mM, c apiosylrhododendrin = 17.2 mM, γ RAPIDASE = 20.0 mg mL –1 , γ β-Glu = 2.0 mg mL –1 , (C) c betuloside = 45.5 mM, c apiosylrhododendrin = 84.6 mM, γ RAPIDASE = 100.0 mg mL –1 , (D) c betuloside = 51.3 mM, c apiosylrhododendrin = 98.0 mM, γ RAPIDASE = 100.0 mg mL –1 , γ β-Glu = 2.0 mg mL –1 . Legend: ● betuloside concentration in experiment, ○ apiosylrhododendrin concentration in experiment, rhododendrol concentration in experiment, – – – betuloside concentration by model, ····· apiosylrhododendrin concentration by model, - - - rhododendrol concentration by model.

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques: Concentration Assay

Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Hydrolysis of crude betuloside ( T = 40 °C; 100 mM phosphate buffer, pH 6). Correlation of experimental data and model through changes in the concentration of betuloside and rhododendrol over time in (A) repetitive batch reactor ( c betuloside = 5 × 62.3 mM, γ β-Glu = 4 mg mL –1 ), (B) batch reactor with a higher substrate concentration ( c betuloside = 275.0 mM, γ β-Glu = 4 mg mL –1 ), and (C) ultrafiltration membrane reaction ( c betuloside = 62.3 mM, γ β-Glu = 4 mg mL –1 , q v = 180 μL min –1 ). Legend: ● betuloside concentration in experiment, ○ rhododendrol concentration in experiment, – – – betuloside concentration by model, - - - rhododendrol concentration by model.

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques: Concentration Assay, Membrane

Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Dependence of process metrics on enzyme concentrations for a mixture of rhododendrol glycosides ( x betuloside = 0.5, c rhod. glyc . = 300 mM). The results for each enzyme’s initial concentration were taken at the time when X rhod.glyc . = 99%. (A) Conversion of rhododendrol glycosides, (B) productivity, (C) final rhododendrol concentration (in g L –1 ), (D) biocatalyst yield for RAPIDASE, and (E) biocatalyst yield for β-glucosidase from almonds.

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques: Concentration Assay

Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Optimal initial concentrations of the enzymes RAPIDASE and β-glucosidase for the hydrolysis of rhododendrol glycoside mixtures ( c 0 = 300 mM), ensuring a substrate conversion of 99% within 24 h determined by simulation.

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques:

Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Estimated Kinetic Parameter, Kinetic Model, and Reactor Models for Rhododendrol Glycoside Hydrolysis Catalyzed by β-Glucosidase from Almonds

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques:

Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Estimated Inactivation Constants and Productivity for Rhododendrol Glycoside Hydrolysis Catalyzed by β-Glucosidase from Almonds in Different Reactors

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques: Membrane

Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Estimated Kinetic Parameters, Kinetic Models, and Reactor Models for Rhododendrol Glycoside Hydrolysis Catalyzed by RAPIDASE

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques:

Journal: ACS Sustainable Chemistry & Engineering

Article Title: Optimization of Biocatalytic Rhododendrol Production from Biogenic Rhododendrol Glycosides

doi: 10.1021/acssuschemeng.4c05889

Figure Lengend Snippet: Estimated Inactivation Constants for Rhododendrol Glycoside Hydrolysis Catalyzed by β-Glucosidase from Almonds and Polygalacturonase from RAPIDASE in a Batch Reactor

Article Snippet: Rhododendrol (racemic mixture) was purchased from TCI (Japan).

Techniques: